Plantlet Regeneration and Multiple Shoot Induction from Protocorm-Like Bodies (PLBs) of Medicinal Orchid Species, Dendrobium crumenatum Sw.

To investigate the suitable medium for in vitro shoot regeneration and plantlet growth of Dendrobium crumenatum Sw., individual protocorm-like bodies (PLBs) (about 4 - 5 mm diameter) Sw. derived from MS supplemented with 0.5 mg/L TDZ 60 days culture were cultured on 6 media; Murashige Skoog (MS) medium, 15 % (v/v) CW, CW 0.2 (w/v) AC, Vacin Went (VW) VW AC. After months culture, containing coconut water (CW) gave highest percentage shooting number shoots per explant 96.0 9.5, respectively a significant difference other media. The addition activated charcoal (AC) significantly increased leaves roots. PLBs developed into complete plantlets. AC roots root length at 5.3 34.9 mm, respectively. transfer rooted to greenhouse, 95 regenerated plantlets survived grew vigorously. Plantlets grown successfully acclimatized greenhouse showed normal development.